Highly mature cardiac marker expression
Our cardiomyocytes expresses voltage-dependent K+ channel genes (HERG, KCNQ1, Kir1.2), and the expression levels of our cardiomyocytes are higher than that of other iPSC-derived CMs. Our cardiomyocytes has relatively mature electrophysiological properties.
Mature muscle fibers
Immunostaining of αActinin clearly showed sarcomere structures.
Our cells are more into organotypic sarcomere formation.
Our cardiomyocytes shows higher activity of mitochondria compared with the conventional CMs.
Myoridge cardiomyocytes have high purity without cell-sorting procedures. The ratio of cardiomyocytes is from 93% to 98%.
In addition, the undifferentiated iPSC ratio is very low.
■Cardiac troponin (cTnT)-positive cell ratio is high (93 – 98%)
■Different Myoridge CM lines show similar cTNT expression
■Oct-4 (pluripotency marker) expression of cardiac colonies
Undiffentiated iPSC (Oct4+) ratio is very low (≪0.01%)