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CarmyA

CarmyA (human iPS cell-derived cardiomyocytes, monkey iPS cell-derived cardiomyocytes)

ur iPS cell-derived cardiomyocytes, CarmyA, are high-quality cardiomyocytes produced by means of protein-free differentiation induction method using our proprietary compounds.

Product information

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Product name Product code Content Storage
CarmyA
(Human iPS cell-derived cardiomyocytes)
H-011106 1.0×10⁶cells/vial Frozen at ultra-low temperature
H-011107 1.0×10⁷cells/vial Frozen at ultra-low temperature
Thawed CarmyA
(Human iPS cell-derived cardiomyocytes)
H-013256 2.5×10⁶cells/flask Live cell
H-013506 5.0×10⁶cells/flask Live cell
CarmyA-GCaMP
(Human iPS cell-derived cardiomyocytes)
G-011106 1.0×10⁶cells/vial Frozen at ultra-low temperature
G-011107 1.0×10⁷cells/vial Frozen at ultra-low temperature
Thawed CarmyA-GCaMP
(Human iPS cell-derived cardiomyocytes)
G-013256 2.5×10⁶cells/flask Live cell
G-013506 5.0×10⁶cells/flask Live cell
CarmyA-Monkey
(Monkey iPS cell-derived cardiomyocytes)
M-011106 1.0×10⁶cells/vial Frozen at ultra-low temperature
CarmyA cardiomyocyte
preserving medium
ME-01A00241 40mL Frozen
CarmyA cardiomyocyte seeding medium kit (10-mL type) ME-02A00211 播種用2倍濃縮培地(2TM)10mL、
播種用基礎培地(TB)10 mL
Frozen

Proprietary sensor protein-expressing products lineup

1. GCaMP-expressing myocardium

Drug discovery requires a high-throughput experimental system. Also, the need for long-term drug exposure testing has increased. Cardiomyocytes with the homeostatic expression of GCaMP, a calcium sensor, enable a measurement of calcium transient with multi well plates and an analysis of the beating patterns after compounds are added. Cells can be observed non-invasively for a long time, because staining with indicators is not required.

Myocardium expressing a calcium sensor protein, GCaMP
Myocardium expressing a calcium sensor protein, GCaMP

2. MaLion-expressing myocardium

An ATP sensor (mito-MaLion) that enables non-invasive live imaging of ATP in mitochondria was developed by Dr. Kitaguchi, Tokyo Institute of Technology, and Dr. Tsuboi, the University of Tokyo. We focused on the usefulness of this sensor protein and generated cardiomyocytes expressing mito-MaLion (CarmyA-mito-MaLion) in a joint research project. CarmyA-mito-MaLion will contribute to drug discovery research and pathological mechanism research.
Please contact us for the generation of various cells expressing mito-MaLion other than cardiomyocytes and the use of other sensor proteins.

Cardiomyocytes expressing mito-MaLion (CarmyA-mito-MaLion), an ATP sensor protein
Cardiomyocytes expressing mito-MaLion (CarmyA-mito-MaLion), an ATP sensor protein

Features

  • High myocardium purity

    CarmyA is produced by means of a chemically defined culture, in which proteins such as cytokines, growth factors, or animal serum components are not used during cardiomyocyte differentiation. This method can provide a stable cell production and relatively high myocardium purity (>90%).

  • Well-developed myofibers

    With the use of floating 3D culture, more cardiomyocytes in CarmyA have distinctly patterned sarcomere structure compared to those with 2D culture, which suggests that the cells are structurally matured.

    More cardiomyocytes in CarmyA are structurally matured.
  • Maturation gene expression

    CarmyA expresses channel genes that are important for pharmacological response and membrane potential; thus a good pharmacological response can be detected. In addition, the medium was improved to facilitate the expression of maturation marker genes.

    Expression of βMHC (matured myocardia/skeletal muscle)
  • Mitochondrial activity

    Energy supply from mitochondria is important for cardiomyocytes because they must keep beating. An increase in the copy number of mitochondria has been observed in a new medium that we developed. The myocardium using this medium have an improved oxygen consumption and activated mitochondria compared with the conventional myocardium.

    Measurement of the oxygen consumption rate (OCR, nmol/minute) of cells

    The maximum respiration can be measured with an addition of carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP). The following figure suggests that mitochondria in CarmyA are highly active.

    CarmyAのミトコンドリア活性図解

References

  • Mita M, Sugawara I, Harada K, Ito M, Takizawa M, Ishida K, Ueda H, Kitaguchi T, Tsuboi T.
    Development of red genetically encoded biosensor for visualization of intracellular glucose dynamics.
    Cell Chem Biol. 2021 Jun 29.
  • Harada K, Chihara T, Hayasaka Y, Mita M, Takizawa M, Ishida K, Arai M, Tsuno S, Matsumoto M, Ishihara T, Ueda H, Kitaguchi T, Tsuboi T.
    Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging.
    Sci Rep. 2020 Nov 11.
  • Kadota S, Minami I, et al., Development of a reentrant arrhythmia model in human pluripotent stem cell-derived cardiac cell sheets. European Heart Journal. 2013 April 34.
  • Minami I, et al., A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Reports, 2012 Nov 29.