Culture medium optimization
What is medium optimization?
Critical factor in cell culture is the medium.
We propose medium optimazation plan using our unique medium components library for issues in
cell culture such as cell phenotype, reproducibility of cell culture, safty and cost of medium,
Myoridge's medium optimization flow achieves maximum performance.
- Optimal medium conditions for each cell line
- Control cell phenotypes
- Medium components and additives suitable for the assay system
Benefits of Myoridge's medium development service
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Utilizing our unique culture medium component library, we propose custom culture media in response to user issues.
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Based on our original serum-free medium, we can develop a medium that excludes specific components such as animal-derived components and expensive growth factors.
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Achieve medium optimization in a short period of time (minimum 2 to 3 months) with high-throughput screening using medium library plates.
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Disclosure of medium composition is also available as an option.
Medium optimization
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Our original
serum-free medium1 Ex-All in One
Serum-free medium that indicates stable proliferation for various cells.
2 Ex-MSC Medium-2
Serum-free medium that indicates stable proliferation, high expression of surface marker for mesenchymal stem cells and maintaining cell morphology. It can be used for regenerative medicine research.
Proposal example
If you are a mesenchymal stem cell user, we propose Ex-MSC Medium-2, or if you are using other cells, we propose Ex-All in One. If the cell culture results are positive, you can purchase the product. If medium optimization is required, we recommend the Step 2 medium kit. Depending on the cells you are using, we will suggest a culture medium kit from the beginning rather than a product.
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Medium kit
We create and provide media kits with several types of media compositions。
Proposal example
We ask you to tell us about your cell type, cell culture issues, and the performance you would like to achieve through culture medium optimization, and we will propose a culture medium kit of some types to 20 types. We will share the cell culture results with you and proceed with the next culture medium optimization cycle.
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Medium library plates
We provide 60 to 80 types of media compositions per plate, allowing for HTS* type media screening. It will come true. It can be expected to shorten the time until culture medium optimization and increase the probability of success.
*HTS: High throughput screeningProposal example
When performing high-throughput media screening, we propose a media screening plate using a 96-well plate. 1 well is 1 composition of medium, it is possible to study medium with more than 60 compositions per plate.
Example of proposal for culture medium optimization service
Exploration of culture conditions using serum-free, low-protein medium (cell function control, differentiation induction method construction, culture cost reduction, etc.)
Based on our original serum-free medium, we propose medium library plates and medium kits to solve the cell culture problems faced by users. Run the medium optimization cycle to search for the optimal medium composition using indicators such as cell proliferation, marker expression, and functionality. Serum-free culture is expected to eliminate variations due to FBS lot differences, suppress adsorption of test substances and cell secretions to serum proteins, and reduce interference with analysis and measurement systems.
faq
- What is the proposal-based media development service?
- In general, custom culture media were manufactured on a made-to-order contract basis, with the customer specifying the culture media components (addition or deletion of components, concentration of components, etc.). Our media development service is a next generation of service in which our researchers examine the optimal culture conditions for each customer's cells and propose deliverables. Our culture screening technology enables us to propose optimal culture conditions (e.g., growth potential, low cost, markers, etc.) for each customer's cells.
- What are the benefits of a proposal-based mediadevelopment service?
- Our proposal-based medium development service provides the best medium for each cell by adding or deleting low-molecular compounds based on our Ex medium (xeno-free, serum-free). This has the advantage of eliminating expensive cytokines and growth factors used in conventional cell culture, as well as serum, which can cause reproducibility (lot-to-lot) problems. Please contact us if you have any problems with cells that are difficult to grow, cost reduction through mass culture, or reproducibility.
- How much cost savings can I expect from the proposed medium development service?
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Cost reduction varies greatly depending on the cell type and culture conditions. We will discuss cost reduction targets with you after confirming your requirements. As an example, we have achieved a cost reduction of 1/10 by replacing cytokines, animal serum proteins, and protein factors with low-molecular-weight compounds and amino acids.
Please tell us about your experiencewith proposal-based culture medium development services.
We have experience in various modifications of culture medium components while maintaining the equivalence of cell proliferation and expression markers, including improvements such as the absence or reduced use of cytokines and other protein components (e.g., serum, FGF-2, insulin, transferrin, albumin). We also have experience with iPS cells, mesenchymal stem cells, and T cells. We also provide a stable supply in large-volume packaging (500 ml) for companies and academic facilities.
- Is it possible to sign a Non-Disclosure Agreement (NDA)?
- Yes, we are willing to sign a non-disclosure agreement upon request.
- How do I place an order?
- Orders and deliveries are made through our distributors. Please contact our Sales Promotion Department for information on our distributors.
- Can you provide powdered culture media?
- We are providing liquid medium only.
