PRODUCTS

Original culture media

Basal Medium for Custom-order, Ex-1 (basal medium for cell growth)

Basal Medium for Custom-order, Ex-1 (basal medium for cell growth) is available for sale. Various cells, such as mesenchymal stem cells, fibroblasts, Chinese hamster ovary (CHO) cells, established T cell lines, and hybridoma B cells, show a stable proliferative capability in this medium.
With our culture-medium development service, we search for and propose highly-efficient, low-cost media that are the best fit for your cells with a variety of techniques, including those for a reduction in expensive protein ingredients, using small-molecular compounds for a high-throughput medium screening.
On the basis of Basal Medium for Custom-order, Ex-1 (basal medium for cell growth), culture-medium screening can be performed by adding or eliminating the appropriate medium components.

Product information

Product code ME-09A00112
Product name Basal Medium for Custom-order, Ex-1 (basal medium for cell growth)
Storage Medium, 4℃; Supplement, -25℃
Content Medium, 100 mL x 1 bottle; Supplement, 4mL x 1 bottle
Price 5,500 yen*

* Excluding tax.
* Shipping costs charged separately according to the destination.

Because this medium was designed for internal use to perform culture-medium screening, it is only available in a 100-mL bottle as a standard product. Please contact us if you are satisfied with the outcome obtained from this product as a medium, and consider making it a regular purchase. We would recommend a custom-order bottle in a special size (e.g., a 500-mL bottle). The price per volume of a larger bottle can be reduced compared to that of the 100-mL bottle.

Features

  • Xeno-free, serum-free basal medium for general use.
  • Successfully reduced the need for expensive protein ingredients required for general cell culture, by adding several small-molecular compounds with our proprietary technique.
  • Stable proliferative capability demonstrated by various cells, such as mesenchymal stem cells, fibroblast, CHO cells, established T cell lines, and hybridoma B cells, which is comparable to that in the media used as a control.
  • Quality has been tested for sterility, endotoxin, and mycoplasma negativity.

On the basis of Basal Medium for Custom-order, Ex-1, culture-medium screening can be performed suitable for your development products.

Physical property

Appearance Red liquid
pH 7.2 to 7.8

Cell growth capacity

Micrograph of cells cultured in Basal Medium for Custom-order, Ex-1, two days after seeding

Micrograph of cells cultured in Basal Medium for Custom-order, Ex-1, two days after seeding

Cell population doubling level of cells cultured in Basal Medium for Custom-order, Ex-1

MSC, Fb, and CHO grow stably in our product.

Cell population doubling level of cells cultured in Basal Medium for Custom-order, Ex-1

In addition to these cells shown above, established T cell lines, several types of hybridoma B cells, and adipose or umbilical cord-derived MSC have been confirmed to grow stably.

Safety data

The karyotypic analysis (chromosome aberration analysis) of MSC cultured in Basal Medium for Custom-order, Ex-1 was performed by means of quantitative polymerase chain reaction. As a result, no alterations were found after 5 and 10 passages.

How to use

Add the supplement to the medium and warm this mixture to the culture temperature (assumed to be approx. 37C) before use. Once you have evaluated the growth outcome and the quality of the cells cultured in this product, we would like to discuss culture-medium development in our laboratory.
A variety of medium compositions other than this product mentioned above are available for culture testing. Please feel free to contact us if you are interested in trying them.

【Steps to use Basal Medium for Custom-order, Ex-1】
Step 1: Use this product to culture your target cells and evaluate the results in your laboratory.*
Step 2: Our laboratory conducts culture-medium screening suitable for your target cells and delivers the outcome.
Step 3: You will receive a continuous supply of completed custom-made media exclusive for your cells from us or our partner company.

* If there are any issues in the cell proliferation or marker expression found in cells cultured with this product, we can provide you with our screening kits that are available for several screening patterns. Please inform us of the cell types, culture conditions, and application (e.g., developing for regenerative medicine products) of the cells. We would accordingly like to choose and send an appropriate screening kit on the basis of provided information to you.

Documents

  • MSDS
    en
  • Product specifications
    en

Please contact us individually if you need analysis reports for each lot.

See below for more information on Cell products
>About Cell products