Development support of in vitro models
Examples of achievements
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Pharmaceutical company
Development of heart failure model using iPS cell-derived cardiomyocytes.
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Pharmaceutical company
Differentiation Induction of cardiomyocytes from genome-edited iPS cells.
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Drug discovery start up company
Development of mitochondrial ATP sensor-introduced cells and drug screening method targeting mitochondria.
Products of iPS cells-derived Cardiomyocyte
Human and monkey iPS cells-derived cardiomyocyte
iPS cells-derived cardiomyocytes CarmyA® are high-quality cardiomyocytes produced using a protein-free differentiation induction method using unique compounds. We have achieved a troponin (cTnT) positivity rate of over 90% in various iPS cells using a protein-free myocardial differentiation induction method. CarmyA® is a highly purified cardiomyocyte with mature sarcomere structure and high mitochondrial activity. Furthermore, maturation process we developed increases the expression of genes specific to ventricular muscle, providing improved sensitivity in drug responsiveness such as Ca2+ transients.
| Product name | CarmyA® | |
|---|---|---|
| Product code | H-011106X | H-011107X |
| Storage |
Under -70 degrees Celsius (long term storage：Under -150 degrees Celsius) |
|
| Volume | 1.0×10⁶ cells | 1.0×10⁷ cells |
| Price | ||
| SDS | ||
| Manual | ||
| Product name | CarmyA®-GCaMP | |
|---|---|---|
| Product code | G-011106 | G-011107 |
| Storage |
Under -70 degrees Celsius (long term storage：Under -150 degrees Celsius) |
|
| Volume | 1.0×10⁶ cells | 1.0×10⁷ cells |
| Price | ||
| SDS | ||
| Manual | ||
| Product name | CarmyA®-Monky | |
|---|---|---|
| Product code | M-011106X | |
| Storage |
Under -70 degrees Celsius (long term storage：Under -150 degrees Celsius) |
|
| Volume | 1.0×10⁶ cells | |
| Price | ||
| SDS | ||
| Manual | ||
| Product name | CarmyA® cardiomyocyte maintenance medium UG |
|
|---|---|---|
| Product code | ME-01A00241 | |
| Storage |
Maintenance medium：-20℃ Supplement：-20℃ |
|
| Volume | CarmyA® cardiomyocyte maintenance medium UG 40 mL | Supplement for maintenance medium UG 1 tube |
| Price | 10,700JPY | |
| SDS | ||
| Manual | ||
| Product name | CarmyA® cardiomyocyte maintenance and seeding kit |
|
|---|---|---|
| Product code | ME-12A001KT | |
| Storage |
Seeding medium：-20℃ Maintenance medium：-20℃ Supplement：-20℃ |
|
| Volume | CarmyA® cardiomyocyte seeding medium-2TM 10 mL | Supplement for seeding medium-2TM 1 tube |
| Price | 21,600JPY | |
| SDS | ||
| Manual | ||
| Product name | Product code | Storage | Volume | Price | SDS | Manual |
|---|---|---|---|---|---|---|
| CarmyA® | H-011106X |
Under -70 degrees Celsius (long term storage：Under -150 degrees Celsius) |
1.0×10⁶ cells | |||
| H-011107X | 1.0×10⁷ cells | |||||
|
CarmyA®- GCaMP |
G-011106 |
Under -70 degrees Celsius (long term storage：Under -150 degrees Celsius) |
1.0×10⁶ cells | |||
| G-011107 | 1.0×10⁷ cells | |||||
| CarmyA®- Monky |
M-011106X |
Under -70 degrees Celsius (long term storage：Under -150 degrees Celsius) |
1.0×10⁶ cells | |||
| CarmyA® cardiomyocyte maintenance medium UG |
ME-01A00241 |
Maintenance medium：-20℃ Supplement：-20℃ |
CarmyA® cardiomyocyte maintenance medium UG 40 mL | 10,700JPY | ||
| Supplement for maintenance medium UG 1 tube | ||||||
|
CarmyA® cardiomyocyte maintenance and seeding kit |
ME-12A001KT |
Seeding medium：-20℃ Maintenance medium：-20℃ Supplement：-20℃ |
CarmyA® cardiomyocyte seeding medium-2TM 10 mL | 21,600JPY | ||
| Supplement for seeding medium-2TM 1 tube | ||||||
| CarmyA® cardiomyocyte seeding medium-TB 10 mL | ||||||
| CarmyA® cardiomyocyte maintenance medium UG 40 mL | ||||||
| Supplement for maintenance medium UG 1 tube |
※CarmyA®, CarmyA®-GCaMP and CarmyA®-Monky are build-to-order manufacturing. Please contact us for delivery dates and prices.
Differentiation induction contract service
We receive your iPS cells derived from patient, healthy or genome edited, and produce and supply iPS cells-derived cardiomyocyte.
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Step
1Evaluation of differentiation directivity- Detail
- Evaluation of Troponin T positive rate 2 weeks after differentiation induction started.
- Deadline
- 1 to 1 and a half months after seeding iPS cells.
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Step
2Examination of differentiation induction conditions and drug responsiveness- Detail
- Calculate yield of differentiated cells each iPS cell line, and examine drug responsiveness.
- Deadline
- 2 to 2 and a half months after seeding iPS cells.
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Step
3Produce and supply iPS cells-derived cardiomyocyte- Detail
- Produce and supply iPS cells-derived cardiomyocyte accoding to yeild of differentiated cells each iPS cell line.
- Deadline
- 2 months after seeding iPS cells.
Sensor protein introduced cells such as MaLion
An ATP sensor (mito-MaLion) that enables non-invasive live imaging of ATP in mitochondria was developed by Dr. Kitaguchi, Tokyo Institute of Technology, and Dr. Tsuboi, the University of Tokyo. We focused on the usefulness of this sensor protein and generated cardiomyocytes expressing mito-MaLion (CarmyA-mito-MaLion) in a joint research project. CarmyA-mito-MaLion will contribute to drug discovery research and pathological mechanism research. Please contact us for the generation of various cells expressing mito-MaLion other than cardiomyocytes and the use of other sensor proteins.
Mito-MaLion Expression in iPS cells-derived cardiomyocyte.
Effect for mito-MaLion brightness value by anti-mitochondrial drug
Production of cell line expressing gene such as MaLion
We receive your cells such as iPS cells, and introduce sensor protein gene into cells using gene editting or plasmid vector. After that we suply cells confirmed fluorescence.
Development of in vitro models by culture medium optimization
We support in vitro model development by our culture medium optimization which control phenotype of cells and explore medium comportment, additive suitable for cell line or assay model.
Cell culture
Cell line and assay model
Various cell screening systems
At Myoridge, we evaluate culture medium components and new culture materials using the cell lines listed below. Our professional team of cell culture can perform cell assays for drug discovery, cosmetics, and functional foods. We can also contract your company's screening by transferring the technology of your in vitro test system.
| cell name | evaluation |
|---|---|
| iPSC | pluripotent, differentiation potency, mass culture・making protocol of automatic culture |
| MSC | cell proliferation, surface marker, chemotaxis, IL-7・transferrin depending cell proliferation activity |
| HUVEC | cell proliferation, surface marker, chemotaxis, angiogenic potency |
| Fibroblast | cell proliferation, surface marker, KGF・FGF depending cell proliferation activity |
| MCF-7 | IGF-1・insulin depending cell proliferation activity |
| RPMI 1788 |
IL-1β depending cell proliferation activity |
| THP-1 | Flt3-L depending cell proliferation activity |
| Reh | Flt3-L depending cell proliferation activity |
| TF-1 | LIF・GM-CSF・IL-4・IL-6・SCF・EPO depending cell proliferation activity |
| CTLL2 | IL-2・IL-5 depending cell proliferation activity |
| M-NFS-60 | G-CSF depending cell proliferation activity |
